Milky supply and weaning

There's one question I've always had. And it's one question for which nobody would give me a straight answer. Basically, what happens to milk supply when you cut out one feed? Does your milk supply decrease in a general way or do you just have less milk for that particular feeding? I got different mixed up answers. A midwife at my ob/gyn practice told me that milk will decrease overall, rather than a no-supply situation during that dropped feeding time.

Why am I wondering about this? I am thinking about cutting out one pumping session at work so I only have to pump once. This would happen once Sam can eat a full meal at the babysitter. But then what happens on weekends when we are together and I'd like to nurse him whenever? I don't find my body in tune with the time of day it is when it comes to nursing. Like I don't feel full on weekends at the times I'd pump if I was at work.

The way it all works is a mystery to me!

I have cut out a feed at night and didn't notice a decrease in supply or anything because Sam just ate more at the next feed. See, I nurse him at bedtime at around 8:30 (all times are "-ish") and then at 11:30 before I go to bed. Then he'd wake up at 1:30-2 for some reason. Not hunger. But I'd feed him then. And then at 5. And then in the morning whenever he'd wake up, 7-8. I decided to cut out that 2am feed because I know he's not hungry 2 hours after a decent feed. Sam would cry at that time, my husband would give him the pacifier and shush him back to sleep, and that was that. He'd wake up at 5 for a feed and eat very nicely because it's been a while since his last meal. He'd never eat well at the 2am feed because he wasn't even hungry. By now our night schedule transformed to one nighttime wake-up sometime in the 4 range, instead of crying at 2 and eating at 5.